Biological dan Molecular Characterization of Papaya Ringspot Virus from Bogor District, Indonesia
Abstract
Management of PRSV using cross-protection and transgenic plants has been hampered due to varying PRSV gene sequences. Therefore, the characterization of new PRSV isolates could help design the region needed for region-specific management practices. The study aimed to characterize the typical PRSV isolates found in Bogor biologically and molecularly. The study was conducted in 2 stages: host range study of 2 isolates (Sukaraja and Cijeruk) on five species with six plants for replication, and RT-PCR analysis amplified coat protein (CP) region using PRSV326 and PRSV800 primer pair. The results showed that Sukaraja isolate produced systemic symptoms in papaya (Carica papaya L) cv. Merah Delima in the form of leaf lamina becoming pale and wilting, leaf malformation, open veins, and striped patterns on the leaves, while the Cijeruk isolate causes symptoms of leaf blistering, mosaicism, leaf malformation, wilted lamina, and striped patterns on the leaves. These two isolates did not cause symptoms on eggplant (Solanum melongena) and chickpea (Vigna unguiculata) but produced striped leaf patterns and pale lamina symptoms on bitter melon (Momordica charantia) and melon (Cucumis melo) leaves. RT-PCR analysis was able to amplify ±475 bp of DNA. The two DNA isolates had a homology percentage of 97.7% with PRSV isolates from Thailand, ranging between 93.07-99.68% with the Kulon Progo and Nganjuk isolates. Based on phylogenetic analysis, the Cijeruk isolate was in the same branch as the Indonesian isolate, while the Sukaraja isolate was a separate branch and closely related to isolates from Oklahoma and Malaysia.
Downloads
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Please find the rights and licenses in Jurnal Proteksi Tanaman (JPT).
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License.
2. Author(s)’ Warranties
The author(s) warrants that the article is original, written by a stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary permissions to quote from other sources have been obtained by the author(s).
3. User Rights
JPT adopts the spirit of open access and open science, which disseminates articles published as free as possible under the Creative Commons license. JPT permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JPT to distributing works in the journal.
4. Rights of Authors
Authors retain the following rights:
- Copyright, and other proprietary rights relating to the article, such as patent rights,
- the right to use the substance of the article in future own works, including lectures and books,
- the right to reproduce the article for own purposes, provided the copies are not offered for sale,
- the right to self-archive the article.
5. Co-Authorship
If other authors jointly prepared the article; upon submitting the article, the author is agreed on this form and warrants that he/she has been authorized by all co-authors to enter into this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement. JPT will be freed on any disputes that will occur regarding this issue.
6. Termination
This agreement can be terminated by the author or JPT upon two months’ notice where the other party has materially breached the conditions set forth in this agreement and failed to remedy such breach within a month of being given the terminating party’s notice requesting such breach to be remedied. No breach nor violation of this agreement will cause this agreement or any license granted in it to terminate automatically or affect the definition of JPT.
7. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permitted, the author waives his or her right to collect royalties relative to the article.
8. Miscellaneous
JPT will publish the article (or have it published) in the journal if the article’s editorial process is completed and JPT or its sublicensee has become obligated to have the article published. JPT may adjust the article to a style of punctuation, spelling, capitalization, referencing, and usage that it deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible, and such access will be free of charge for the readers.