Control of Fusarium fujikuroi Cause of Bakanae Disease in Rice by Trichoderma harzianum Culture Filtrate
English
Abstract
Trichoderma harzianum culture filtrate is widely used to control plant pathogenic fungi because it contains secondary metabolites which act as antifungal. This study aimed to determine the ability of T. harzianum culture filtrate at various concentrations to suppress the growth of Fusarium fujikuroi cause of bakanae disease in rice. The study consisted of 2 stages: 1. in the laboratory and 2. in the screen house using a Completely Randomized Design (CRD) consisting of 6 treatments and 5 replications. The treatments were T. harzianum culture filtrate concentrations, namely A, 0%, B. 25%, C. 50%, D.75%, E. 100% and F. control (without filtrate and without F. fujikuroi). Parameters observed for stage I (in the laboratory) i.e. the percentage of seeds attacked by F. fujikuroi, colony thickness and colony area and parameters observed for stage 2 (in the screen house) i.e. the number of seedlings appearing, the seeds showing symptoms of bakanae, dead seeds, dead seedlings and stunting seeds. The results showed that the T. harzianum culture filtrate was able to suppress the growth of F.fujikuroi at both in the laboratory and in the screen house. The best filtrate concentration was 100% with the inhibition on the colony area of 77.38%, the number of seeds germinating of 55.78%, seeds showing bakanae of 80.06%, dead seeds of 60.09%, dead seedlings of 52.97%, and stunting seedlings of 60.09%
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